USPTO Patents Application 10570220

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CLAIMS

1 . A humanized anti-TAG-72 CC49 monoclonal antibody comprising:
5 a light chain comprising a light chain Complementarity Determining Region

(L-CDR)1, a L-CDR2, a L-CDR3, and a light chain framework region from
HuCC49V10, a heavy chain comprising a heavy chain Complementarity
Determining Region (H-CDR)1, a H-CDR2, a H-CDR3, and a heavy chain
framework region from HuCC49V10, wherein the light chain framework region

1 0 comprises a corresponding framework residue from human antibody LEN at
position 5, 19, 21, and 106 in the light chain, and wherein the heavy chain
framework region comprises a Corresponding framework residue from human
antibody 21/28'CL at positions 20, 38, 48, 66, 67, 69, and 80 in the heavy chain;

wherein the humanized CC49 antibody retains binding affinity for TAG-72

15 and has reduced immunogenicity, as compared to a parental humanized CC49 V10
antibody.

2. The monoclonal antibody of claim 1, further comprising a corresponding
human LEN framework residue at position 43 in the light chain.

20

3. The monoclonal antibody of claim 1, further comprising a corresponding
human LEN framework residue at position 78 in the light chain.

4. The monoclonal antibody of claim 1, further comprising a corresponding
25 LEN human framework residue at position 100 in the light chain.

5. The monoclonal antibody of claim 1, further comprising a corresponding
human 21/28'CL framework residue at position 12 in the heavy chain.

30 6. The monoclonal antibody of claim 1, further comprising a corresponding

human 21/28'CL framework residue at position 40 in the heavy chain.

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7. The monoclonal antibody of claim 1, wherein the light chain framework
region further comprises a corresponding human LEN framework residue at position
43, 78, and 100 in the light chain and a corresponding human 21/28'CL framework
residue at position 12 in the heavy chain.

8. The monoclonal antibody of claim 1, wherein L-CDR1 comprises an
amino acid sequence set forth as SEQ ID NO: 9, L-CDR2 comprises an amino acid
sequence set forth as SEQ ID NO: 10, and L-CDR3 comprises an amino acid
sequence set forth as SEQ ID NO: 1 1 .

9. The monoclonal antibody of claim 1, wherein H-CDR1 comprises an
amino acid sequence et forth s SEQ ID NO: 12, H-CDR2 comprises an amino acid
sequence set forth as SEQ ID NO: 13, and H-CDR3 comprises an amino acid
sequence set forth as SEQ ID NO: 14.

10. The monoclonal antibody of claim 1, wherein the light chain framework
comprises SEQ ID NO: 1 and wherein the residue at position 5 in the light chain is a
threonine, the residue at position 19 in the light chain is an alanine, and the residue
at position 21 in the light chain is an isoleucine.

11. The monoclonal antibody of claim 1, wherein the light chain framework
comprises an amino acid sequence set forth as SEQ ID NO: 2 and wherein the
residue at position 43 in the light chain is a proline.

25 12. The monoclonal antibody of claim 1, wherein the light chain framework

comprises an amino acid sequence set forth as SEQ ID NO: 3 and wherein the
residue at position 78 in the light chain is a leucine.

13. The monoclonal antibody of claim 1, wherein the light chain framework
30 comprises an amino acid sequence set forth as SEQ ID NO: 4 and wherein the

residue at position 100 in the light chain is a glutamine and the residue at position
106 in the light chain is an isoleucine.

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14. The monoclonal antibody of claim 1, wherein the heavy chain
framework comprises an amino acid sequence set forth as SEQ ID NO: 5 and
wherein residue at position 20 in the heavy chain is a valine.

5

15. The monoclonal antibody of claim 14, wherein the heavy chain
framework comprises an amino acid sequence set forth as SEQ ID NO: 5 and
wherein residue at position 12 in the heavy chain is a lysine.

10 16. The monoclonal antibody of claim 1, wherein the heavy chain

framework comprises an amino acid sequence set forth as SEQ ID NO: 6 and
wherein the residue at position 38 in the heavy chain is an arginine and the residue at
position 48 in the heavy chain is a methionine.

15 17. The monoclonal antibody of claim 1 6, wherein the heavy chain

framework comprises an amino acid sequence set forth as SEQ ID NO: 6 and
wherein the residue at position 40 in the heavy chain is an alanine.

18. The monoclonal antibody of claim 1, wherein the heavy chain
20 framework comprises an amino acid sequence set forth as SEQ ID NO: 7 and

wherein the residue at position 66 in the heavy chain is an arginine, the residue at
position 67 in the heavy chain is an isoleucine, and the residue at position 80 in the
heavy chain is a methionine.

25 1 9. The monoclonal antibody of claim 1 , wherein the heavy chain

framework comprises the amino acid sequence set forth as SEQ ID NO: 8.

20. The monoclonal antibody of claim 1, further comprising a detectable

label.

30

21. The monoclonal antibody of claim 1, further comprising an effector
molecule.

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22. The monoclonal antibody of claim 20, wherein the label is a fluorescent
or a radioactive molecule.

5 23. The monoclonal antibody of claim 21, wherein the effector molecule is a

toxin.

24. A composition comprising a functional fragment of the humanized
monoclonal antibody of claim 1, wherein the functional fragment specifically binds

10 TAG-72.

25. The composition of claim 24, wherein the fragment comprises an Fv, an
Fab, or an F(ab'>2.

15 26. The composition of claim 25, wherein the antibody is encoded by a

nucleic acid sequence as deposited as ATCC PTA-5415.

27. A pharmaceutical composition comprising a therapeutically effective
amount of the antibody of claim 1 in a pharmaceutically acceptable carrier.

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28. A method for treating a subject with a tumor that expresses TAG-72,
comprising: administering a therapeutically effective amount of the humanized
antibody of claim 1 to the subject, thereby treating the tumor.

25 29. The method of claim 28, wherein the humanized antibody is encoded by

a nucleic acid sequence as deposited as ATCC PTA-5415.

30. A method for detecting a cell expressing TAG-72 in a subject,
comprising

30 contacting a sample from the subject with the antibody of claim 1, and

detecting the presence of a complex of the antibody with TAG-72,
thereby detecting a cell expressing TAG-72.

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3 1 . The method of claim 30, wherein the subject has a tumor.

32. The method of claim 31, wherein the antibody is labeled.

33. The method of claim 30, wherein the antibody is encoded by a nucleic
acid deposited as ATCC PTA-5415.

34. The method of claim 30, wherein the sample is a biopsy specimen,
autopsy specimen, and pathology specimens, or a biological fluid.

35. A method for in vivo diagnosis of cancer in a subject, comprising

(a) administering to an mammal a diagnostically effective amount of the
antibody of claim 20,

(b) allowing sufficient time for the antibody to become specifically localized
to at least one cancer cell, and

(c) detecting the labeled antibody in vivo at a site where the antibody has
become localized, thereby diagnosing the cancer.

20 36. A kit comprising

a container comprising the humanized antibody of claim 1 and
instructions.

37. A monoclonal antibody, comprising a heavy and a light chain variable
25 region, wherein

the light chain variable region comprises a light chain framework region
comprising amino acid sequences set forth as SEQ ID NOs: 41-44, and light chain
complementarity determining regions comprising amino acid sequences set forth as
SEQ NOs: 9-12;

30 the heavy chain variable region comprises a heavy chain framework region

comprising amino acid sequences set forth as SEQ ID NOs: 49-52, and heavy chain

5

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complementarity determining regions comprising amino acid sequences set forth as
SEQ ID NOs: 12-14; and

wherein the humanized CC49 antibody retains binding affinity for TAG-72
and has reduced immunogenicity, as compared to a parental humanized CC49 VI 0
5 antibody.

38. An isolated nucleic acid encoding the antibody of claim 1 .

39. A vector comprising a promoter operably linked to the nucleic acid of
10 claim 38.

40. A host cell transformed with the vector of claim 39.

41. The host cell of claim 40, wherein the cell is a eukaryotic cell.

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42. The antibody of claim 1, further comprising a tyrosine to proline
substitution in L-CDR3 at position 91.

43. The antibody of claim 42, further comprising a valine to leucine
20 substitution at position 27b.